Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content

Bitter leaf, Vernonia amygdalina (VA), is used in various alternative medicine several countries, including Indonesia. This study aims to evaluate the antioxidant activity of VA leaves by three different vitro methods. The was evaluated radical scavenging 1,1-diphenyl-2- picryl-hydrazyl (DPPH), ferric reducing power (FRAP), and cupric capacity (CUPRAC). FRAP CUPRAC methods were expressed as mg quercetin equivalents (QE) per g extract (mgQE/g) ascorbic acid (AAE) (mgAAE/g), respectively. All measured UV-Visible spectrophotometry at maximum wavelength. results exhibited that ethanolic contains flavonoidphenolic. has an DPPH method with half inhibition concentration (IC50) 8,22 μg/mL. 21,03 mgQE/g 160,64 mgAAE/g extract, a powerful potency which might correlate flavonoid-phenolic content extract.